Use this NAD+ calculator to turn a vial of lyophilised NAD+, your bacteriostatic water volume, and a dose in milligrams into the exact mL and units to draw. NAD+ is a coenzyme rather than a peptide, but injectable vials are reconstituted and measured exactly the same way. This page covers the math, not dosing decisions.
- Common vial examples
- 100 / 500 / 1000 mg
- Example dose
- 50 mg
- Common syringe
- U-100 insulin
- Last reviewed
Quick math example
A 500 mg vial mixed with 5 mL gives 100 mg/mL. A 50 mg dose is 0.5 mL, which equals 50 units on a U-100 syringe.
NAD+ reconstitution reference
U-100 units to draw for each common NAD+ dose, by vial size, reconstituted with 5 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 25 mg | 50 mg | 100 mg |
|---|---|---|---|---|
| 500 mg | 100 mg/mL | 25 u(0.25 mL) | 50 u(0.5 mL) | 100 u(1 mL) |
| 1000 mg | 200 mg/mL | 12.5 u(0.13 mL) | 25 u(0.25 mL) | 50 u(0.5 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
How the NAD+ calculation works
NAD+ is dosed in milligrams — usually tens of milligrams per injection — rather than the micrograms used for most peptides, and vials are large (100 to 1000 mg). Concentration is the vial mg divided by the water volume; the draw is your dose divided by that concentration.
Because the numbers are large, it is easy to slip a decimal place. Entering the vial, water and dose separately, as the calculator does, keeps mg, mL and units lined up.
- 500 mg vial = 500,000 mcg total
- 500 mg / 5 mL = 100 mg/mL
- 50 mg / 100 mg per mL = 0.5 mL = 50 U-100 units
Why 100 mg/mL is the common NAD+ concentration
At 100 mg/mL every milligram of NAD+ is exactly 0.01 mL, which is one unit on a U-100 insulin syringe. A 25 mg dose is 25 units, 50 mg is 50 units and 100 mg is a full 100-unit syringe, so the dose you want is the number you read.
That is why 500 mg vials are usually mixed with 5 mL and 1000 mg vials with 10 mL. If your vial cannot hold that much water, use half the volume: the concentration doubles to 200 mg/mL and every draw halves.
- 100 mg/mL → 1 mg = 1 unit on a U-100 syringe
- 200 mg/mL → 1 mg = 0.5 units (50 mg = 25 units)
- 50 mg/mL → 1 mg = 2 units (50 mg = 100 units)
How much BAC water for a 100 mg NAD+ vial
A 100 mg vial in 1 mL gives 100 mg/mL: 25 mg = 25 units, 50 mg = 50 units, and the whole vial is 100 units. In 2 mL it is 50 mg/mL and every draw doubles (25 mg = 50 units, 50 mg = 100 units).
A 100 mg vial holds four 25 mg doses or two 50 mg doses, so it suits trying a protocol before buying a larger vial.
- 100 mg / 1 mL = 100 mg/mL → 25 mg = 25 units, 50 mg = 50 units
- 100 mg / 2 mL = 50 mg/mL → 25 mg = 50 units, 50 mg = 100 units
- Four 25 mg doses or two 50 mg doses per vial
NAD+ 100 mg vial: units per dose (1 mL BAC water)
U-100 units to draw for each common NAD+ dose, by vial size, reconstituted with 1 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 25 mg | 50 mg | 100 mg |
|---|---|---|---|---|
| 100 mg | 100 mg/mL | 25 u(0.25 mL) | 50 u(0.5 mL) | 100 u(1 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
How much BAC water for a 500 mg NAD+ vial
5 mL is the usual choice for a 500 mg vial because it gives 100 mg/mL, where 1 mg = 1 unit: 25 mg = 25 units, 50 mg = 50 units, 100 mg = 100 units. With 2.5 mL the vial is 200 mg/mL and each draw halves (50 mg = 25 units).
A 500 mg vial holds ten 50 mg doses or twenty 25 mg doses. Check the vial's fill capacity before adding 5 mL, and that the reconstituted beyond-use date covers how long those doses will take you.
- 500 mg / 5 mL = 100 mg/mL → 50 mg = 0.5 mL = 50 units
- 500 mg / 2.5 mL = 200 mg/mL → 50 mg = 0.25 mL = 25 units
- About ten 50 mg doses per vial
NAD+ 500 mg vial: units per dose (5 mL BAC water)
U-100 units to draw for each common NAD+ dose, by vial size, reconstituted with 5 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 25 mg | 50 mg | 100 mg |
|---|---|---|---|---|
| 500 mg | 100 mg/mL | 25 u(0.25 mL) | 50 u(0.5 mL) | 100 u(1 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
How much BAC water for a 1000 mg NAD+ vial
A 1000 mg vial in 10 mL gives 100 mg/mL, the same easy 1 mg = 1 unit reading as 500 mg in 5 mL. If the vial only holds 5 mL, you get 200 mg/mL, where 50 mg is 25 units and 100 mg is 50 units.
A 1000 mg vial holds about twenty 50 mg doses. At one or two doses a week that is longer than most reconstituted beyond-use windows, so many people choose a smaller vial or confirm the date with their pharmacy before mixing.
- 1000 mg / 10 mL = 100 mg/mL → 50 mg = 50 units
- 1000 mg / 5 mL = 200 mg/mL → 50 mg = 25 units, 100 mg = 50 units
- Plan around the beyond-use date, not just the dose count
NAD+ 1000 mg vial: units per dose (10 mL BAC water)
U-100 units to draw for each common NAD+ dose, by vial size, reconstituted with 10 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 25 mg | 50 mg | 100 mg |
|---|---|---|---|---|
| 1000 mg | 100 mg/mL | 25 u(0.25 mL) | 50 u(0.5 mL) | 100 u(1 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
Injection volume and comfort
Injected NAD+ is widely reported to cause temporary flushing, warmth or stinging, which is one reason doses are often kept modest and volumes small. How much and how fast is a question for your prescriber or pharmacy; the calculator's job is making sure the volume you draw matches the dose you intend.
If a dose computes to more than 1 mL, it will not fit a standard insulin syringe. Use a stronger concentration (less water), split the dose, or use a larger syringe, then re-run the math.
Frequently asked questions
Is NAD+ a peptide?
How much BAC water should I add to a 500 mg NAD+ vial?
How many units is 50 mg of NAD+?
How many doses are in a 1000 mg NAD+ vial?
Does this calculator tell me what NAD+ dose to take?
Primary sources
Background references for this calculator. PepSync does not make clinical claims; these citations support the educational context only.