Use this MOTS-c calculator to convert vial strength and BAC water volume into a precise draw amount. The page focuses on math, not on dosing recommendations.
- Common vial examples
- 5 / 10 / 40 mg
- Example dose
- 5 mg
- Common syringe
- U-100 insulin
- Last reviewed
Quick math example
A 10 mg vial mixed with 2 mL gives 5 mg/mL. A 5 mg dose is 1.0 mL, which equals 100 units on a U-100 syringe.
MOTS-c reconstitution reference
U-100 units to draw for each common MOTS-c dose, by vial size, reconstituted with 2 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 2.5 mg | 5 mg | 10 mg |
|---|---|---|---|---|
| 5 mg | 2.5 mg/mL | 100 u(1 mL) | 200 u(2 mL) | 400 u(4 mL) |
| 10 mg | 5 mg/mL | 50 u(0.5 mL) | 100 u(1 mL) | 200 u(2 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
How the MOTS-c calculation works
MOTS-c is typically labelled in milligrams per vial. The calculator first divides the vial mg amount by your BAC water volume to find concentration, then divides your target dose by that concentration.
Once concentration is known, every dose becomes target mg divided by mg per mL.
- 10 mg vial = 10,000 mcg total
- 10,000 mcg / 2 mL = 5,000 mcg per mL
- 5,000 mcg target = 1.0 mL draw
MOTS-c 5 mg vial examples
A 5 mg vial in 1 mL gives 5 mg/mL. A 2.5 mg dose is 0.5 mL (50 units). Adding more water lowers the concentration: 5 mg in 2 mL gives 2.5 mg/mL, so a 2.5 mg dose becomes a full 1.0 mL (100 units).
That is why 5 mg vials are often mixed with less water than 10 mg vials — it keeps a multi-milligram dose inside a 1 mL syringe.
- 5 mg / 1 mL = 5 mg/mL → 2.5 mg = 0.5 mL = 50 units
- 5 mg / 2 mL = 2.5 mg/mL → 2.5 mg = 1.0 mL = 100 units
- A 5 mg vial holds about two 2.5 mg doses
MOTS-c 5 mg vial: units per dose (1 mL BAC water)
U-100 units to draw for each common MOTS-c dose, by vial size, reconstituted with 1 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 2.5 mg | 5 mg | 10 mg |
|---|---|---|---|---|
| 5 mg | 5 mg/mL | 50 u(0.5 mL) | 100 u(1 mL) | 200 u(2 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
MOTS-c 10 mg vial with 2 mL example
A 10 mg MOTS-c vial mixed with 2 mL gives 5 mg/mL, or 5,000 mcg/mL.
A 5 mg dose is 5,000 mcg, which at this concentration is a full 1.0 mL — 100 units, the entire length of a standard 1 mL U-100 syringe. A 2.5 mg dose would be half that: 0.5 mL, or 50 units.
- 10 mg / 2 mL = 5 mg/mL
- 5 mg / 5 mg per mL = 1.0 mL = 100 U-100 units
- 2.5 mg / 5 mg per mL = 0.5 mL = 50 U-100 units
MOTS-c 10 mg vial: units per dose (2 mL BAC water)
U-100 units to draw for each common MOTS-c dose, by vial size, reconstituted with 2 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 2.5 mg | 5 mg | 10 mg |
|---|---|---|---|---|
| 10 mg | 5 mg/mL | 50 u(0.5 mL) | 100 u(1 mL) | 200 u(2 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
How much BAC water for a 40 mg MOTS-c vial
A 40 mg vial holds four times the peptide of a 10 mg vial, so at the same water volume it is four times as concentrated. In 2 mL it gives 20 mg/mL, so a 5 mg dose is 0.25 mL, or 25 units on a U-100 syringe.
If you want the more familiar draw sizes from a 10 mg vial, use proportionally more water: 8 mL on a 40 mg vial gives 5 mg/mL, so a 5 mg dose returns to 1 mL (100 units). 4 mL is a common middle ground at 10 mg/mL (5 mg = 50 units). Check the vial can hold the volume, and that the beyond-use date covers the eight or so 5 mg doses it contains.
- 40 mg / 2 mL = 20 mg/mL → 5 mg = 0.25 mL = 25 units
- 40 mg / 4 mL = 10 mg/mL → 5 mg = 0.50 mL = 50 units
- 40 mg / 8 mL = 5 mg/mL → 5 mg = 1.0 mL = 100 units
MOTS-c 40 mg vial: units per dose (4 mL BAC water)
U-100 units to draw for each common MOTS-c dose, by vial size, reconstituted with 4 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 2.5 mg | 5 mg | 10 mg |
|---|---|---|---|---|
| 40 mg | 10 mg/mL | 25 u(0.25 mL) | 50 u(0.5 mL) | 100 u(1 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
Reduce the draw with less water or a stronger vial
Because MOTS-c doses are in the multi-milligram range, a dilute vial can push a single dose to the full syringe. Reconstituting with less water raises the concentration and shrinks the draw.
The same 10 mg vial in 1 mL gives 10 mg/mL, so a 5 mg dose drops to 0.5 mL (50 units) instead of a full mL. Always enter your actual figures so the units match your setup.
- 10 mg / 1 mL = 10 mg/mL, so a 5 mg dose = 0.5 mL = 50 units
- Less BAC water = higher concentration = smaller draw
- Keep the draw within your syringe's capacity
Frequently asked questions
Is MOTS-c approved as a medicine?
Why does my MOTS-c draw volume look large?
Can a MOTS-c dose exceed my syringe capacity?
Can PepSync save a MOTS-c protocol?
How much BAC water for a 40 mg MOTS-c vial?
Primary sources
Background references for this calculator. PepSync does not make clinical claims; these citations support the educational context only.