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MOTS-c reconstitution & dosage calculator

Mix a 10 mg MOTS-c vial with 2 mL of bacteriostatic water to get 5 mg/mL. A 5 mg dose is then 1 mL, which is 100 units on a U-100 insulin syringe.

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  • Formula shown below
  • U-100 syringe units

MOTS-c dose calculator

Free, no CC requiredSafely follow your doses and schedules.Get PepSyncGet appResearch from Built with research from Harvard, Oxford & WHO
On the vial label
mg
Amount you add
mL
What you plan to take
Syringe sizeU-100 insulin syringe
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Built with research from
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  • University of Oxford
  • Harvard Medical School

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How your MOTS-c draw is calculated

  1. 1Find the concentration

    10 mg × 1,000 ÷ 2 mL
    = 5,000 mcg/mL

  2. 2Work out the volume

    5,000 mcg ÷ 5,000 mcg/mL
    = 1.00 mL

  3. 3Convert to units

    1.00 mL × 100
    = 100 units

On a U-100 insulin syringe, 100 units equals 1 mL, so each unit is 0.01 mL. The 0.3 mL (30 unit) and 0.5 mL (50 unit) syringes use the same scale, just shorter, which makes small doses easier to read.

Use this MOTS-c calculator to convert vial strength and BAC water volume into a precise draw amount. The page focuses on math, not on dosing recommendations.

Common vial examples
5 / 10 / 40 mg
Example dose
5 mg
Common syringe
U-100 insulin
Last reviewed

Storage before and after reconstitution: Lyophilised 2–8°C; reconstituted vial refrigerated and protected from light.

Educational reference only. Not medical advice — follow the instructions from your clinician or pharmacy.

Quick answers: MOTS-c doses in units

With a 10 mg vial mixed with 2 mL of bacteriostatic water (5 mg/mL), on a U-100 insulin syringe:

How many units is 2.5 mg of MOTS-c?
50 units (0.5 mL)
How many units is 5 mg of MOTS-c?
100 units (1 mL)
How many units is 10 mg of MOTS-c?
200 units (2 mL), more than one 1 mL syringe

Educational maths only, not a dose recommendation. Use the calculator above for your own vial, water volume and syringe.

Quick math example

A 10 mg vial mixed with 2 mL gives 5 mg/mL. A 5 mg dose is 1.0 mL, which equals 100 units on a U-100 syringe.

MOTS-c reconstitution reference

U-100 units to draw for each common MOTS-c dose, by vial size, reconstituted with 2 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.

VialConcentration2.5 mg5 mg10 mg
5 mg2.5 mg/mL100 u(1 mL)200 u(2 mL)400 u(4 mL)
10 mg5 mg/mL50 u(0.5 mL)100 u(1 mL)200 u(2 mL)

Swipe the table to see every dose →

Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.

MOTS-c units chart: U-100 syringe units for each common dose in 5 and 10 mg vials mixed with 2 mL of bacteriostatic water
MOTS-c units chart: the table above as one image to save or share.

MOTS-c dosage chart: units for 10 mg and 40 mg vials

MOTS-c is dosed in milligrams, not micrograms, which makes it one of the larger draws among research peptides. Commonly cited amounts run from about 2.5 mg to 10 mg per injection, given a few times a week, often in cycles of several weeks. MOTS-c is not an approved medicine and human dosing data are limited, so this page does not recommend a dose. The chart shows what each commonly cited amount looks like on a U-100 syringe.

Because the doses are large, concentration decides whether a dose fits in one syringe. At 5 mg/mL (10 mg in 2 mL) a 10 mg dose is 2 mL — two full 1 mL syringes. At 10 mg/mL (10 mg in 1 mL, or 40 mg in 4 mL) the same dose is a single 100-unit draw. The 40 mg column shows the same concentration as 10 mg in 1 mL but four times as many doses per vial.

  • 10 mg + 2 mL (5 mg/mL): 2.5 mg = 50 units · 5 mg = 100 units
  • 10 mg + 1 mL (10 mg/mL): 2.5 mg = 25 units · 5 mg = 50 units · 10 mg = 100 units
  • 40 mg + 4 mL (10 mg/mL): eight 5 mg doses per vial

MOTS-c dosage chart in U-100 units

Dose10 mg + 2 mL5 mg/mL10 mg + 1 mL10 mg/mL40 mg + 4 mL10 mg/mL
1 mg20 u(0.2 mL)10 per vial10 u(0.1 mL)10 per vial10 u(0.1 mL)40 per vial
2.5 mg50 u(0.5 mL)4 per vial25 u(0.25 mL)4 per vial25 u(0.25 mL)16 per vial
5 mg100 u(1 mL)2 per vial50 u(0.5 mL)2 per vial50 u(0.5 mL)8 per vial
7.5 mg150 u(1.5 mL)1 per vial · over 1 mL75 u(0.75 mL)1 per vial75 u(0.75 mL)5 per vial
10 mg200 u(2 mL)1 per vial · over 1 mL100 u(1 mL)1 per vial100 u(1 mL)4 per vial

Swipe the table to see every vial →

Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100); “per vial” is how many of that dose the vial holds, before the small dead-space loss on each draw. Confirm against your own vial label, diluent, and clinician or pharmacy instructions.

How the MOTS-c calculation works

MOTS-c is typically labelled in milligrams per vial. The calculator first divides the vial mg amount by your BAC water volume to find concentration, then divides your target dose by that concentration.

Once concentration is known, every dose becomes target mg divided by mg per mL.

  • 10 mg vial = 10,000 mcg total
  • 10,000 mcg / 2 mL = 5,000 mcg per mL
  • 5,000 mcg target = 1.0 mL draw

MOTS-c 5 mg vial examples

A 5 mg vial in 1 mL gives 5 mg/mL. A 2.5 mg dose is 0.5 mL (50 units). Adding more water lowers the concentration: 5 mg in 2 mL gives 2.5 mg/mL, so a 2.5 mg dose becomes a full 1.0 mL (100 units).

That is why 5 mg vials are often mixed with less water than 10 mg vials — it keeps a multi-milligram dose inside a 1 mL syringe.

  • 5 mg / 1 mL = 5 mg/mL → 2.5 mg = 0.5 mL = 50 units
  • 5 mg / 2 mL = 2.5 mg/mL → 2.5 mg = 1.0 mL = 100 units
  • A 5 mg vial holds about two 2.5 mg doses

MOTS-c 5 mg vial: units per dose (1 mL BAC water)

U-100 units to draw for each common MOTS-c dose, by vial size, reconstituted with 1 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.

VialConcentration2.5 mg5 mg10 mg
5 mg5 mg/mL50 u(0.5 mL)100 u(1 mL)200 u(2 mL)

Swipe the table to see every dose →

Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.

MOTS-c 10 mg vial with 2 mL example

A 10 mg MOTS-c vial mixed with 2 mL gives 5 mg/mL, or 5,000 mcg/mL.

A 5 mg dose is 5,000 mcg, which at this concentration is a full 1.0 mL — 100 units, the entire length of a standard 1 mL U-100 syringe. A 2.5 mg dose would be half that: 0.5 mL, or 50 units.

  • 10 mg / 2 mL = 5 mg/mL
  • 5 mg / 5 mg per mL = 1.0 mL = 100 U-100 units
  • 2.5 mg / 5 mg per mL = 0.5 mL = 50 U-100 units

MOTS-c 10 mg vial: units per dose (2 mL BAC water)

U-100 units to draw for each common MOTS-c dose, by vial size, reconstituted with 2 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.

VialConcentration2.5 mg5 mg10 mg
10 mg5 mg/mL50 u(0.5 mL)100 u(1 mL)200 u(2 mL)

Swipe the table to see every dose →

Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.

How much BAC water for a 40 mg MOTS-c vial

A 40 mg vial holds four times the peptide of a 10 mg vial, so at the same water volume it is four times as concentrated. In 2 mL it gives 20 mg/mL, so a 5 mg dose is 0.25 mL, or 25 units on a U-100 syringe.

If you want the more familiar draw sizes from a 10 mg vial, use proportionally more water: 8 mL on a 40 mg vial gives 5 mg/mL, so a 5 mg dose returns to 1 mL (100 units). 4 mL is a common middle ground at 10 mg/mL (5 mg = 50 units). Check the vial can hold the volume, and that the beyond-use date covers the eight or so 5 mg doses it contains.

  • 40 mg / 2 mL = 20 mg/mL → 5 mg = 0.25 mL = 25 units
  • 40 mg / 4 mL = 10 mg/mL → 5 mg = 0.50 mL = 50 units
  • 40 mg / 8 mL = 5 mg/mL → 5 mg = 1.0 mL = 100 units

MOTS-c 40 mg vial: units per dose (4 mL BAC water)

U-100 units to draw for each common MOTS-c dose, by vial size, reconstituted with 4 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.

VialConcentration2.5 mg5 mg10 mg
40 mg10 mg/mL25 u(0.25 mL)50 u(0.5 mL)100 u(1 mL)

Swipe the table to see every dose →

Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.

Reduce the draw with less water or a stronger vial

Because MOTS-c doses are in the multi-milligram range, a dilute vial can push a single dose to the full syringe. Reconstituting with less water raises the concentration and shrinks the draw.

The same 10 mg vial in 1 mL gives 10 mg/mL, so a 5 mg dose drops to 0.5 mL (50 units) instead of a full mL. Always enter your actual figures so the units match your setup.

  • 10 mg / 1 mL = 10 mg/mL, so a 5 mg dose = 0.5 mL = 50 units
  • Less BAC water = higher concentration = smaller draw
  • Keep the draw within your syringe's capacity

Frequently asked questions

What is a typical MOTS-c dosage in units?
This page does not recommend a dose. Commonly cited MOTS-c amounts are 2.5–10 mg per injection, a few times a week. In units it depends on your concentration: from a 10 mg vial in 2 mL (5 mg/mL), 2.5 mg is 50 units and 5 mg is 100 units; in 1 mL (10 mg/mL) those halve to 25 and 50 units.
Is MOTS-c approved as a medicine?
MOTS-c is a mitochondrial-derived research peptide and is not an approved human medicine in the major markets. This page only explains the reconstitution math and makes no treatment recommendation.
Why does my MOTS-c draw volume look large?
MOTS-c doses are typically in the multi-milligram range, so at 5 mg/mL a single 5 mg dose fills a whole 1 mL syringe. Lower the draw by reducing BAC water or using a higher-strength vial.
Can a MOTS-c dose exceed my syringe capacity?
Yes — a dilute vial and a large target can compute to more than 1 mL, which will not fit a standard insulin syringe. If that happens, reconstitute with less water to raise the concentration, then re-run the math.
Can PepSync save a MOTS-c protocol?
Yes. The app saves vial size, water volume, dose, schedule, and reminders together so the calculator inputs do not need to be re-entered each cycle.
How much BAC water for a 40 mg MOTS-c vial?
Scale it to the draw you want. 2 mL gives 20 mg/mL (a 5 mg dose = 25 units); 4 mL gives 10 mg/mL (5 mg = 50 units); 8 mL gives 5 mg/mL (5 mg = 100 units). More water lowers concentration and raises the units for the same dose.

Primary sources

Background references for this calculator. PepSync does not make clinical claims; these citations support the educational context only.

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