Formula methodology
PepSync calculators use dimensional conversion only. They assume the vial amount, diluent volume, target dose, and syringe type entered by the user are correct, then convert those values into concentration, mL to draw, syringe units, and doses remaining.
The core formulas are: 1 mg = 1,000 mcg; concentration = total peptide divided by liquid volume; dose volume = target dose divided by concentration; syringe units = mL multiplied by the syringe scale, such as 100 for a U-100 syringe.
What the calculator does not decide
Product labels, pharmacy instructions, prescribing information, and clinician guidance should override generic educational content. PepSync cannot determine whether a product, diluent, storage window, or dose is appropriate.
The calculator also cannot verify sterility, product identity, compounding quality, storage conditions, expiration dates, individual suitability, or whether a protocol is medically appropriate.
Source categories users should check
For product-specific questions, users should confirm current product labeling, dispensing pharmacy instructions, prescriber or clinician guidance, and applicable regulatory information in their jurisdiction.
For calculator concepts, start with the reconstitution guide, unit conversion guide, and syringe units guide.
Primary references
PepSync's educational content draws on the following regulatory, pharmacopeial, and peer-reviewed references. Each citation links directly to the source.
Reconstitution and vial handling
References used for general vial handling, aseptic technique, and the limits of educational calculator guidance.
- Safe Injection Practices to Prevent Transmission of Infections to PatientsCDC · 2024
Supports cautious language around sterile needles, syringes, medication vials, and contamination prevention.
- Handling and Storage Guidelines for PeptidesBachem · 2024
Supports general peptide handling, lyophilized storage, solution stability, and moisture sensitivity context.
Bacteriostatic water and sterile water
References used for BAC water, benzyl alcohol preservative language, and diluent caution.
- Bacteriostatic Water for Injection, USPDailyMed / National Library of Medicine · 2024
Supports the description of bacteriostatic water as sterile water containing benzyl alcohol preservative.
- Bacteriostatic preserved saline for pain-free periocular injections: reviewPMC / National Library of Medicine · 2022
Provides peer-reviewed context on benzyl alcohol-preserved saline and bacteriostatic properties.
Syringe units and dose math
References used for U-100 unit conversion, medication measurement confusion, and calculator math framing.
- FDA alerts health care providers, compounders and patients of dosing errors associated with compounded injectable semaglutide productsFDA · 2024
Supports the importance of clear mg, mL, and unit conversion around vial-based products.
- Guidance on the Content of Premarket Notification [510(k)] Submissions for Piston SyringesFDA · 1995
Supports U-100 syringe marking context, including standard insulin syringe volumes and labeling.
- HUMALOG prescribing informationDailyMed / National Library of Medicine · 2025
Supports the definition of U-100 as 100 units/mL in a medication-label context.
Storage and beyond-use dates
References used for storage caution, beyond-use date variability, and pharmacy-label-first language.
- USP Compounding Standards beyond-use date fact sheetUSP · 2025
Supports the distinction between expiration dates and beyond-use dates for compounded preparations.
- The Pharmacist Guide to Assigning a Beyond-Use DateASHP · 2025
Supports pharmacy-led BUD assignment and conservative source-label guidance.
GLP-1 medications and vial dosing errors
FDA prescribing information and peer-reviewed trial data for the GLP-1 medications referenced in PepSync's calculator presets.
- FDA alerts health care providers, compounders and patients of dosing errors associated with compounded injectable semaglutide productsFDA · 2024
Supports caution around compounded injectable products, multiple-dose vials, and unit measurement errors.
- Mounjaro (tirzepatide) prescribing informationFDA · 2022
Authoritative tirzepatide dosing, titration, and storage reference.
- Ozempic (semaglutide) prescribing informationFDA · 2022
Authoritative semaglutide dosing, titration schedule, and storage reference.
- Triple-hormone-receptor agonist retatrutide for obesity — a phase 2 trialNew England Journal of Medicine · 2023
Peer-reviewed efficacy data for retatrutide; the dose ranges studied inform what is realistic to track.
GLP-1 dose escalation schedules
The prescribing information and trial publications behind the titration steps on PepSync's GLP-1 dosage chart pages.
- Zepbound (tirzepatide) prescribing informationFDA · 2023
Tirzepatide for weight management: start at 2.5 mg weekly, increase in 2.5 mg steps after at least 4 weeks, maintenance 5, 10 or 15 mg, maximum 15 mg.
- Mounjaro (tirzepatide) prescribing informationFDA · 2022
Tirzepatide for type 2 diabetes: 2.5 mg for 4 weeks, then 5 mg, then 2.5 mg steps after at least 4 weeks on each dose, maximum 15 mg.
- Wegovy (semaglutide) prescribing informationFDA · 2021
Semaglutide for weight management: 0.25 mg weekly for 4 weeks, then 4-week steps through 0.5, 1 and 1.7 mg to the 2.4 mg maintenance dose.
- Ozempic (semaglutide) prescribing informationFDA · 2022
Semaglutide for type 2 diabetes: 0.25 mg for 4 weeks, then 0.5 mg, then optional steps to 1 mg and 2 mg after at least 4 weeks on each dose.
- Saxenda (liraglutide) prescribing informationFDA · 2025
Daily dose escalation 0.6 → 3.0 mg; pen strength 6 mg/mL (18 mg/3 mL).
- Triple-hormone-receptor agonist retatrutide for obesity — a phase 2 trialNew England Journal of Medicine · 2023
Weekly retatrutide groups of 1, 4, 8 and 12 mg; the 12 mg group started at 2 mg and the 4 and 8 mg groups at 2 or 4 mg.
- Retatrutide for obesity, obstructive sleep apnea and knee osteoarthritis: rationale and design of the TRIUMPH trialsDiabetes, Obesity and Metabolism · 2026
Phase 3 design: fixed dose escalation over 16 weeks to weekly maintenance doses of 4, 9 or 12 mg.
SS-31 / elamipretide research literature
Clinical and review literature for SS-31, studied as the drug elamipretide.
- Efficacy and Safety of Elamipretide in Individuals With Primary Mitochondrial Myopathy: The MMPOWER-3 Randomized Clinical TrialNeurology · 2023
Phase 3 trial of subcutaneous elamipretide at 40 mg a day for 24 weeks; it did not improve the primary outcomes.
- Elamipretide: A Review of Its Structure, Mechanism of Action, and Therapeutic PotentialInternational Journal of Molecular Sciences · 2025
Review of the peptide's cardiolipin-binding mechanism and the conditions it has been studied in.
KPV research literature
Preclinical literature for the melanocortin-derived tripeptide KPV (Lys-Pro-Val).
- Melanocortin-derived tripeptide KPV has anti-inflammatory potential in murine models of inflammatory bowel diseaseInflammatory Bowel Diseases · 2008
Mouse colitis models; the evidence for KPV is preclinical, with no completed human efficacy trials.
- Orally Targeted Delivery of Tripeptide KPV via Hyaluronic Acid-Functionalized Nanoparticles Efficiently Alleviates Ulcerative ColitisMolecular Therapy · 2017
Oral nanoparticle delivery of KPV in mice, the basis for oral KPV products.
BPC-157 research literature
Peer-reviewed reviews on BPC-157 cited as background for the BPC-157 calculator. PepSync does not make clinical claims; the calculator simply converts vial setup into dose volume.
- Stable gastric pentadecapeptide BPC 157 and wound healingPubMed / Frontiers in Pharmacology · 2018
Review of BPC-157 mechanisms in inflammation and tissue healing contexts.
- Brain-gut axis and pentadecapeptide BPC 157PubMed / Current Pharmaceutical Design · 2016
Reference review covering BPC-157's reported physiological effects.
TB-500 / Thymosin Beta-4 research literature
Peer-reviewed references for thymosin beta-4 (commonly sold as TB-500) cited as background for the TB-500 calculator.
- Thymosin β4 and cardiac protection: implication in inflammation and fibrosisPubMed / Annals of the New York Academy of Sciences · 2012
Reference review on thymosin beta-4 mechanisms relevant to repair contexts.
- Thymosin beta 4: a multi-functional regenerative peptidePubMed / Expert Opinion on Biological Therapy · 2012
Background review on thymosin beta-4 in wound and tissue repair.
CJC-1295 and Ipamorelin research literature
Peer-reviewed pharmacokinetic and pharmacodynamic references for the CJC-1295 and Ipamorelin compounds referenced in the calculator presets.
- Pharmacokinetic and pharmacodynamic studies with CJC-1295, a long-acting growth hormone releasing peptidePubMed / Journal of Clinical Endocrinology & Metabolism · 2006
Foundational pharmacokinetic data for CJC-1295.
- Ipamorelin, the first selective growth hormone secretagoguePubMed / European Journal of Endocrinology · 2001
Background reference on ipamorelin as a selective GHRH secretagogue.
Tesamorelin (Egrifta SV and Egrifta WR)
FDA labels for the two approved tesamorelin products, used for vial mixing, dose volume and storage after reconstitution.
- EGRIFTA SV (tesamorelin for injection) prescribing informationDailyMed / National Library of Medicine · n.d.
2 mg vial mixed with 0.5 mL sterile water; 1.4 mg once daily; inject immediately after mixing.
- EGRIFTA WR (tesamorelin for injection) prescribing informationDailyMed / National Library of Medicine · 2025
11.6 mg vial mixed with 1.3 mL bacteriostatic water (8 mg/mL); 1.28 mg once daily; discard 7 days after mixing.
IGF-1 LR3 (LONG R3 IGF-I)
Manufacturer and supplier datasheets for reconstituting and storing LONG R3 IGF-I.
- LONG R3 IGF-I frequently asked questionsRepligen · n.d.
Manufacturer guidance: reconstitute with 100 mM acetic acid to 1 mg/mL.
- R3 IGF-1 (ab58738) datasheetAbcam · n.d.
Lyophilised from 0.1 M acetic acid; reconstitute in 10 mM HCl to 1 mg/mL; store frozen.
ARA-290 (cibinetide) clinical trials
Published phase 2 trials of ARA-290, used for the trial doses cited on the calculator page.
- ARA 290 improves symptoms in patients with sarcoidosis-associated small nerve fiber lossPMC / Molecular Medicine · 2013
4 mg under the skin once daily for 28 days (Dahan et al.).
- ARA 290, a nonerythropoietic peptide engineered from erythropoietin, improves metabolic control and neuropathic symptoms in patients with type 2 diabetesPMC / Molecular Medicine · 2014
4 mg self-injected under the skin daily for 28 days, 0.5 mL per injection (Brines et al.).
- Cibinetide improves corneal nerve fiber abundance in patients with sarcoidosis-associated small nerve fiber lossPubMed / Investigative Ophthalmology & Visual Science · 2017
Phase 2b: 1, 4 or 8 mg a day vs placebo for 28 days; only 4 mg reached significance (Culver et al.).
Human chorionic gonadotropin (hCG) for injection
FDA labels and guidance used for hCG vial sizes, mixing volumes, storage after mixing and the doses cited on the HCG calculator.
- NOVAREL (chorionic gonadotropin for injection) prescribing informationDailyMed / National Library of Medicine · 2023
5,000 and 10,000 USP unit vials supplied with 10 mL bacteriostatic water; refrigerate after mixing and use within 30 days.
- PREGNYL (chorionic gonadotropin for injection) prescribing informationDailyMed / National Library of Medicine · 2025
10,000 USP unit vial; add 1–10 mL of the supplied solvent; stable 60 days refrigerated after mixing.
- Evaluation and Management of Testosterone Deficiency (AUA guideline)American Urological Association · 2018
hCG is an option for men with testosterone deficiency who want to preserve fertility (conditional recommendation).
- Concomitant intramuscular human chorionic gonadotropin preserves spermatogenesis in men undergoing testosterone replacement therapyPubMed / Journal of Urology · 2013
500 IU every other day alongside TRT in a small retrospective series (Hsieh et al.).
Somatropin (HGH) units and storage
Labels used for the 3 IU = 1 mg somatropin conversion and storage after reconstitution.
- Humatrope (somatropin) prescribing informationRxList (FDA label text) · n.d.
5 mg vial = 15 IU; 6, 12 and 24 mg cartridges = 18, 36 and 72 IU (3 IU per mg).
- Genotropin storage and handlingPfizer Medical Information · n.d.
Reconstituted cartridges with preserved diluent: refrigerate, up to 28 days.
Kisspeptin (kisspeptin-10 and kisspeptin-54)
Human and preclinical studies of kisspeptin's effect on the reproductive (hypothalamic-pituitary-gonadal) axis. Kisspeptin is a research compound, not an approved medicine.
- Kisspeptin-54 stimulates the hypothalamic-pituitary gonadal axis in human malesPubMed / Journal of Clinical Endocrinology & Metabolism · 2005
First human study: peripheral kisspeptin raises LH, FSH and testosterone; context for the compound, not a dosing source.
- Kisspeptin Administration Stimulates Reproductive Hormones but Does Not Affect Anxiety in HumansPubMed / Journal of Clinical Endocrinology & Metabolism · 2025
Recent controlled human administration study of reproductive-hormone effects.
- Central and peripheral administration of kisspeptin-10 stimulates the hypothalamic-pituitary-gonadal axisPubMed / Journal of Neuroendocrinology · 2004
Preclinical study of the kisspeptin-10 fragment sold in research vials.
Semax and Selank research literature
Research on the ACTH(4-10) analogue Semax and the heptapeptide Selank. Both are approved in Russia and treated as research compounds by the FDA and EMA.
- Semax, an analog of ACTH(4-10) with cognitive effects, regulates BDNF and trkB expression in the rat hippocampusPubMed / Brain Research · 2006
Mechanism background for Semax; preclinical.
- Peptide-based Anxiolytics: The Molecular Aspects of Heptapeptide Selank Biological ActivityPubMed / Protein and Peptide Letters · 2018
Review of Selank's proposed mechanisms.
- A comparison of the anxiolytic effect and tolerability of selank and phenazepam in the treatment of anxiety disordersPubMed / Zhurnal Nevrologii i Psikhiatrii imeni S.S. Korsakova · 2014
Clinical comparison from Russia, where Selank is approved; context only.
Safety and regulatory notes
References used for unapproved peptide caution and safety notes on compounds discussed in calculator examples.
- Changes of melanocytic lesions induced by Melanotan injections and sun bed use in a teenage patient with FAMMM syndromePubMed · 2013
Supports cautious wording around melanocytic lesion changes reported after Melanotan 2 use in a case report.
- Melanotan Tanning Injection: A Rare Cause of PriapismPMC / National Library of Medicine · 2021
Supports cautious wording that adverse event reports exist for melanotan II use.
Editorial and review policy
PepSync pages are written to explain calculator mechanics and reduce unit-conversion mistakes. Content is reviewed for internal consistency, clear educational boundaries, and alignment with the formulas shown on this page.
We prioritize updates when calculator behavior changes, when a common search query reveals unclear wording, or when user feedback identifies an error in a worked example.
Correction process
If you spot an error, outdated explanation, or unclear wording, send the page URL, the specific sentence or calculation, and the reason it appears incorrect through the contact page.
Calculation errors are checked against the formula methodology above. If a page is corrected, the page updated date is refreshed and the surrounding example is reviewed for related mistakes.
Useful starting pages
The most important public calculator pages are the peptide reconstitution calculator, peptide dose calculator, and peptide concentration calculator. Dedicated peptide pages, such as semaglutide, tirzepatide, and BPC-157, apply the same formula structure to common peptide-specific examples.