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Per-compound reconstitution
Each compound is calculated on its own — different vials, different concentrations, different draws. Use these references, then run your exact numbers on each compound's calculator.
BPC-157 reference
U-100 units to draw for each common BPC-157 dose, by vial size, reconstituted with 2 mL of bacteriostatic water.
| Vial | Concentration | 200 mcg | 250 mcg | 500 mcg |
|---|---|---|---|---|
| 5 mg | 2.5 mg/mL | 8 u(0.08 mL) | 10 u(0.1 mL) | 20 u(0.2 mL) |
| 10 mg | 5 mg/mL | 4 u(0.04 mL) | 5 u(0.05 mL) | 10 u(0.1 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
TB-500 reference
U-100 units to draw for each common TB-500 dose, by vial size, reconstituted with 2 mL of bacteriostatic water.
| Vial | Concentration | 2 mg | 2.5 mg | 5 mg |
|---|---|---|---|---|
| 2 mg | 1 mg/mL | 200 u(2 mL) | 250 u(2.5 mL) | 500 u(5 mL) |
| 5 mg | 2.5 mg/mL | 80 u(0.8 mL) | 100 u(1 mL) | 200 u(2 mL) |
| 10 mg | 5 mg/mL | 40 u(0.4 mL) | 50 u(0.5 mL) | 100 u(1 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
Wolverine stack dosage chart (pre-mixed BPC-157 + TB-500 vial)
Wolverine stack dosages are usually written per peptide, while a pre-mixed vial is labelled with the total. For a 1:1 vial the blend dose is simply double the per-peptide dose, so "250 mcg of each" means 500 mcg of blend. Community protocols for pre-mixed vials most often cite 500 mcg to 1 mg of blend a day — 250 to 500 mcg of each peptide — for a block of four to six weeks. Neither peptide is an approved medicine and those figures are not from a label, so this page does not recommend one.
The chart reads the blend dose across the three common vial set-ups. Each cell shows the units to draw and how many doses that vial holds, which is what decides when you need the next vial.
- 10 mg (5 + 5) + 2 mL = 5 mg/mL → 500 mcg blend = 10 units
- 10 mg (5 + 5) + 3 mL = 3.33 mg/mL → 500 mcg blend = 15 units
- 20 mg (10 + 10) + 2 mL = 10 mg/mL → 1 mg blend = 10 units
Wolverine stack dosage chart in U-100 units
| DoseEach peptide | 10 mg + 2 mL5 mg/mL | 10 mg + 3 mL3.33 mg/mL | 20 mg + 2 mL10 mg/mL |
|---|---|---|---|
| 500 mcg250 mcg + 250 mcg | 10 u(0.1 mL)20 per vial | 15 u(0.15 mL)20 per vial | 5 u(0.05 mL)40 per vial |
| 750 mcg375 mcg + 375 mcg | 15 u(0.15 mL)13 per vial | 22.5 u(0.22 mL)13 per vial | 7.5 u(0.08 mL)26 per vial |
| 1 mg500 mcg + 500 mcg | 20 u(0.2 mL)10 per vial | 30 u(0.3 mL)10 per vial | 10 u(0.1 mL)20 per vial |
| 2 mg1 mg + 1 mg | 40 u(0.4 mL)5 per vial | 60 u(0.6 mL)5 per vial | 20 u(0.2 mL)10 per vial |
Swipe the table to see every vial →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100); “per vial” is how many of that dose the vial holds, before the small dead-space loss on each draw. Confirm against your own vial label, diluent, and clinician or pharmacy instructions.
See also: BPC-157 dosage chart · TB-500 dosage calculator
How the two schedules overlap
BPC-157 is short-acting and usually run on a daily or twice-daily schedule, while TB-500 is long-acting and often run as a higher weekly loading amount for the first few weeks, then a lower weekly maintenance amount. Because their cadences differ, you calculate and draw each one on its own — never mix them in a single syringe based on a combined number.
A typical overlapping block runs both compounds for 4–6 weeks: BPC-157 daily, TB-500 once or twice weekly. Tracking two reconstitution dates and two doses-remaining counts at once is exactly the kind of thing that gets miscounted on paper.
- Calculate BPC-157 and TB-500 separately — different vials, different concentrations
- BPC-157: daily/EOD; TB-500: weekly loading then weekly maintenance
- Keep two beyond-use dates and two doses-remaining counts in view
Pre-mixed Wolverine blend vials
A pre-mixed Wolverine vial contains both peptides, most commonly 5 mg BPC-157 + 5 mg TB-500 (10 mg total) or 10 mg + 10 mg (20 mg total). Enter the total as the vial amount in the calculator above: the ratio is 1:1, so every draw is half BPC-157 and half TB-500.
A 10 mg vial in 2 mL is 5 mg/mL of blend, so 10 units (0.1 mL) is 500 mcg total — 250 mcg of each peptide. A 20 mg vial in 2 mL is 10 mg/mL, so the same 10 units is 1 mg total, or 500 mcg of each. If your protocol is written per peptide, double it to get the total blend dose for a 1:1 vial.
- 10 mg (5 + 5) / 2 mL = 5 mg/mL → 10 units = 250 mcg BPC-157 + 250 mcg TB-500
- 20 mg (10 + 10) / 2 mL = 10 mg/mL → 10 units = 500 mcg of each
- 1:1 blend: total dose = per-peptide dose × 2
Why you never combine the math
It is tempting to add the two vial amounts together and draw once, but that produces the wrong dose for both compounds. Each vial is reconstituted to its own concentration, so the mL that delivers your TB-500 dose will deliver the wrong amount of BPC-157 and vice versa. The reference tables above show each compound's units independently; that is the correct way to run the stack.
- Two vials = two concentrations = two separate draws
- Combining the totals over-doses one compound and under-doses the other
- If a single blended vial is genuinely co-lyophilised, calculate from its labelled total instead
Frequently asked questions
What is the Wolverine stack dosage in units?
What is the Wolverine stack?
How do I calculate a pre-mixed Wolverine vial?
Can I draw BPC-157 and TB-500 in the same syringe?
What is a common BPC-157 + TB-500 schedule?
How many doses will each vial last for the stack?
Primary sources
Background references for this page. PepSync does not make clinical claims; these citations support the educational context only.
- 1Stable gastric pentadecapeptide BPC 157 and wound healingPubMed / Frontiers in Pharmacology · 2018
- 2Brain-gut axis and pentadecapeptide BPC 157PubMed / Current Pharmaceutical Design · 2016
- 3Thymosin β4 and cardiac protection: implication in inflammation and fibrosisPubMed / Annals of the New York Academy of Sciences · 2012
- 4Thymosin beta 4: a multi-functional regenerative peptidePubMed / Expert Opinion on Biological Therapy · 2012