Quick math example
A 5 mg vial mixed with 2 mL gives 2,500 mcg/mL. A 2.5 mg dose is 1.0 mL, or 100 units on a U-100 syringe.
Reconstitution reference
U100 units to draw for each common TB-500 dose, by vial size, reconstituted with 2 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 2 mg | 2.5 mg | 5 mg |
|---|---|---|---|---|
| 2 mg | 1 mg/mL | 200 u(2 mL) | 250 u(2.5 mL) | 500 u(5 mL) |
| 5 mg | 2.5 mg/mL | 80 u(0.8 mL) | 100 u(1 mL) | 200 u(2 mL) |
| 10 mg | 5 mg/mL | 40 u(0.4 mL) | 50 u(0.5 mL) | 100 u(1 mL) |
Educational reference only — not a dose recommendation. Units assume a U100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
How the TB-500 calculation works
TB-500 is usually labeled in milligrams per vial while target amounts are often discussed in milligrams or micrograms. The calculator keeps the conversions explicit so the syringe reading is unambiguous.
After concentration is known, every dose becomes target mcg divided by mcg per mL.
- 5 mg vial = 5,000 mcg total
- 2 mL water = 2,500 mcg per mL
- 2,500 mcg target = 1.0 mL draw
TB-500 10 mg vial with 3 mL example
A 10 mg TB-500 vial mixed with 3 mL gives about 3,333 mcg/mL, or 3.33 mg/mL.
A 2.5 mg dose is 2,500 mcg, which at this concentration is 0.75 mL — 75 units on a U-100 syringe. Using a larger vial with more water keeps a sizeable dose under the 1 mL mark.
- 10 mg / 3 mL = 3.33 mg/mL
- 2,500 mcg / 3,333 mcg per mL = 0.75 mL
- 0.75 mL x 100 = 75 U-100 units
Stacking and protocol tracking
Recovery protocols often pair TB-500 with BPC-157 on overlapping schedules. Calculate each compound on its own page so the per-draw volume stays separate, then track both vials' reconstitution dates and doses remaining together.
TB-500 is frequently dosed in larger loading amounts up front and lower maintenance amounts later, so saving the setup avoids re-deriving the units each phase.
Frequently asked questions
Is TB-500 approved as a medicine?+
How do I calculate a BPC-157 and TB-500 stack?+
What changes if my TB-500 vial uses more water?+
How does the calculator estimate doses remaining?+
Primary sources
Full reference listBackground references for this calculator. PepSync does not make clinical claims; these citations support the educational context only.
- 1Thymosin β4 and cardiac protection: implication in inflammation and fibrosisPubMed / Annals of the New York Academy of Sciences · 2012
- 2Thymosin beta 4: a multi-functional regenerative peptidePubMed / Expert Opinion on Biological Therapy · 2012