PepSync

Peptide concentration calculator

Use this page to find the concentration of a mixed peptide vial. Concentration is the bridge between vial strength, water volume, target dose, and syringe units.

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  • Formula shown below
  • U-100 syringe units

Peptide dose calculator

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On the vial label
mg
Amount you add
mL
What you plan to take
Syringe sizeU-100 insulin syringe
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jusPeaches · App Store review · United States
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App Store rating
4.9
calculations run
428,000+
peptides supported
100s
Built with research from
  • World Health Organization
  • University of Oxford
  • Harvard Medical School

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How your peptide draw is calculated

  1. 1Find the concentration

    10 mg × 1,000 ÷ 2 mL
    = 5,000 mcg/mL

  2. 2Work out the volume

    500 mcg ÷ 5,000 mcg/mL
    = 0.10 mL

  3. 3Convert to units

    0.10 mL × 100
    = 10 units

On a U-100 insulin syringe, 100 units equals 1 mL, so each unit is 0.01 mL. The 0.3 mL (30 unit) and 0.5 mL (50 unit) syringes use the same scale, just shorter, which makes small doses easier to read.

Core inputs
Vial mg + water mL
Core outputs
mg/mL and mcg/mL
Best for
Checking dilution
Educational reference only. Not medical advice — follow the instructions from your clinician or pharmacy.

How concentration is calculated

Peptide concentration is the amount of peptide divided by the liquid volume after reconstitution. A 10 mg vial in 2 mL is twice as concentrated as a 10 mg vial in 4 mL.

After concentration is known, any target dose can be converted into mL and syringe units using the dose calculator.

  • 10 mg / 2 mL = 5 mg/mL
  • 5 mg/mL = 5,000 mcg/mL
  • More diluent means lower concentration

5 mg vial with 2 mL concentration example

A 5 mg vial mixed with 2 mL has a concentration of 2.5 mg/mL. In micrograms, that is 2,500 mcg/mL.

That concentration means every 0.1 mL contains 250 mcg, which is 10 units on a U-100 syringe.

  • 5 mg / 2 mL = 2.5 mg/mL
  • 2.5 mg/mL = 2,500 mcg/mL
  • 0.1 mL x 2,500 mcg/mL = 250 mcg

10 mg vial with 2 mL concentration example

A 10 mg vial mixed with 2 mL has a concentration of 5 mg/mL. In micrograms, that is 5,000 mcg/mL.

That concentration means every 0.1 mL contains 500 mcg, which is also 10 units on a U-100 syringe.

  • 10 mg / 2 mL = 5 mg/mL
  • 5 mg/mL = 5,000 mcg/mL
  • 0.1 mL x 5,000 mcg/mL = 500 mcg

Why concentration comes first

Unit markings on a syringe only tell you volume. The concentration tells you how much peptide is inside that volume.

Recording concentration alongside reconstitution date and water volume makes future dose checks much easier to audit.

Frequently asked questions

Is mg/mL the same as mcg/mL?
They describe the same concentration in different units. One milligram equals 1,000 micrograms, so 5 mg/mL equals 5,000 mcg/mL.
Does concentration change after the vial is mixed?
The calculated concentration stays based on the original vial amount and final volume, but storage, sterility, and beyond-use dates still matter.
Can I use this for any peptide?
The math works for vial-based concentration calculations, but product-specific instructions and suitability belong with the label, pharmacy, or clinician.
From PepSync users

Dosing with confidence.

  • “Very good and accurate… shows you a diagram of where to pin. Very easy to use and very beginner friendly if you’re starting off.”
    jesaiah685 · App Store review · Australia
  • “User friendly. Easy to navigate and understand.”
    jusPeaches · App Store review · United States
  • “Well put together. Nice friendly layout.”
    Crowleyboy666 · App Store review · United Kingdom
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