Use this AOD-9604 calculator to convert vial strength, BAC water volume, and target dose into the exact amount to draw. AOD-9604 is a modified fragment of growth hormone; this page covers the reconstitution math, not dosing recommendations.
- Common vial examples
- 2 / 5 mg
- Example dose
- 300 mcg
- Common syringe
- U-100 insulin
- Last reviewed
Storage before and after reconstitution: Lyophilised 2–8°C; reconstituted vial refrigerated and protected from light.
Quick answers: AOD-9604 doses in units
With a 5 mg vial mixed with 2 mL of bacteriostatic water (2.5 mg/mL), on a U-100 insulin syringe:
- How many units is 250 mcg of AOD-9604?
- 10 units (0.1 mL)
- How many units is 300 mcg of AOD-9604?
- 12 units (0.12 mL)
- How many units is 500 mcg of AOD-9604?
- 20 units (0.2 mL)
Educational maths only, not a dose recommendation. Use the calculator above for your own vial, water volume and syringe.
Quick math example
A 5 mg vial mixed with 2 mL gives 2,500 mcg/mL. A 300 mcg dose is 0.12 mL, which equals 12 units on a U-100 syringe.
AOD-9604 reconstitution reference
U-100 units to draw for each common AOD-9604 dose, by vial size, reconstituted with 2 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 250 mcg | 300 mcg | 500 mcg |
|---|---|---|---|---|
| 2 mg | 1 mg/mL | 25 u(0.25 mL) | 30 u(0.3 mL) | 50 u(0.5 mL) |
| 5 mg | 2.5 mg/mL | 10 u(0.1 mL) | 12 u(0.12 mL) | 20 u(0.2 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
How the AOD-9604 calculation works
AOD-9604 vials are labelled in milligrams while doses are discussed in micrograms. Concentration is the vial total in mcg divided by your BAC water volume; the draw volume is your target dose divided by that concentration.
Because doses are small, a U-100 insulin syringe keeps the draw readable.
- 5 mg vial = 5,000 mcg total
- 5,000 mcg / 2 mL = 2,500 mcg per mL
- 300 mcg target = 0.12 mL draw
AOD-9604 5 mg vial with 2 mL example
A 5 mg AOD-9604 vial mixed with 2 mL gives 2.5 mg/mL, or 2,500 mcg/mL.
A 300 mcg dose is 0.12 mL (12 units); a 250 mcg dose is 0.1 mL (10 units). A smaller 2 mg vial in 1 mL also gives 2 mg/mL, so match your water volume to your usual dose.
- 5 mg / 2 mL = 2.5 mg/mL → 300 mcg = 0.12 mL = 12 units
- 5 mg / 2 mL = 2.5 mg/mL → 250 mcg = 0.1 mL = 10 units
- 2 mg / 1 mL = 2 mg/mL → 300 mcg = 0.15 mL = 15 units
Reconstitution and storage notes
Bacteriostatic water is common for multi-dose vials. Once reconstituted, keep the vial refrigerated and protected from light, and confirm a beyond-use date with your source. The calculator returns the draw for whatever vial and water amounts you enter.
- Use the diluent your protocol specifies
- Refrigerate the reconstituted vial; protect from light
- Re-run the math whenever vial size, water, or dose changes
AOD-9604 2 mg vial: how much BAC water
A 2 mg AOD-9604 vial with 1 mL of bacteriostatic water gives 2 mg/mL, so 250 mcg is 12.5 units and 500 mcg is 25 units on a U-100 syringe. With 2 mL it is 1 mg/mL, which doubles every draw (250 mcg = 25 units) and makes small doses easier to read.
- 2 mg + 1 mL = 2 mg/mL → 250 mcg = 12.5 units · 500 mcg = 25 units
- 2 mg + 2 mL = 1 mg/mL → 250 mcg = 25 units · 500 mcg = 50 units
AOD-9604 2 mg vial: units per dose (1 mL BAC water)
U-100 units to draw for each common AOD-9604 dose, by vial size, reconstituted with 1 mL of bacteriostatic water. Change the water volume in the calculator above to recompute for your own setup.
| Vial | Concentration | 250 mcg | 300 mcg | 500 mcg |
|---|---|---|---|---|
| 2 mg | 2 mg/mL | 12.5 u(0.13 mL) | 15 u(0.15 mL) | 25 u(0.25 mL) |
Swipe the table to see every dose →
Educational reference only — not a dose recommendation. Units assume a U-100 insulin syringe (100 units = 1 mL on U-100). Always confirm against your own vial, diluent, and clinician or pharmacy instructions.
How to reconstitute AOD-9604 (and whether to use acetic acid)
Wipe both stoppers with alcohol, draw the bacteriostatic water, and let it run slowly down the inside wall of the vial instead of onto the powder. Swirl or roll gently until the solution is clear — do not shake it — then label the vial with the date and the water you added.
Some guides suggest acetic acid for AOD-9604, but no manufacturer or trial source we could find calls for it, and suppliers usually list bacteriostatic water. Note that the published human trials of AOD-9604 gave it into a vein or as an oral tablet, never as an injection under the skin, so there is no trial data for the way it is usually used.
- 5 mg + 2 mL = 2.5 mg/mL → 300 mcg = 12 units
- 2 mg + 1 mL = 2 mg/mL → 300 mcg = 15 units
Frequently asked questions
Is AOD-9604 approved as a medicine?
How much BAC water should I use for an AOD-9604 vial?
Why are AOD-9604 doses entered in mcg?
Do you reconstitute AOD-9604 with acetic acid?
How do I reconstitute a 5 mg AOD-9604 vial?
Primary sources
Background references for this calculator. PepSync does not make clinical claims; these citations support the educational context only.